Inhibition of p65 Nuclear Translocation by KIOM-79 / 대한해부학회지
Korean Journal of Anatomy
;
: 103-110, 2006.
Article
in Korean
| WPRIM
| ID: wpr-656241
ABSTRACT
We demonstrate that KIOM-79, combined extracts isolated from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of RAW 264.7 cells with KIOM-79 inhibited LPS-stimulated nitric oxide production in a doserelated manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65 and EMSA showed that KIOM-79 inhibited NF-kappa/Rel nuclear translocation and DNA binding, respectively. Collectively, this series of experiments indicates that KIOM inhibits iNOS gene expression by blocking NF-kappa/Rel. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of KIOM-79 on iNOS suggest that KIOM-79 may represent a useful anti-inflammatory agent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Gene Expression
/
Negotiating
/
Euphorbia
/
Glycyrrhiza uralensis
/
Pueraria
/
Magnolia
/
Macrophages
/
Nitric Oxide
Language:
Korean
Journal:
Korean Journal of Anatomy
Year:
2006
Type:
Article
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