Aberrant CpG Island Hypermethylation of the RUNX3 Gene in Osteosarcoma / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 285-293, 2009.
Article
in Korean
| WPRIM
| ID: wpr-656420
ABSTRACT
PURPOSE:
Transcriptional silencing of tumor suppressor genes by aberrant methylation of CpG islands plays a crucial role in the development of human cancers. We comprehensively examined the methylation status of several tumor suppressor genes in osteosarcoma with a special focus on the RUNX3 gene. MATERIALS ANDMETHODS:
Methylation-specific polymerase chain reaction (MSP) was performed for osteosarcoma tissues and their cell lines. MSP and RT-PCR for the RUNX3 gene were performed in the tumor-derived cell lines and the immortalized cell lines. The demethylating agent 5-aza-2' deoxycytidine was used in the SaOS-2 cell line to reverse the methylation status.RESULTS:
Hypermethylation of the RUNX3 gene was observed in 60% (24 of 40) of the osteosarcoma tissues, whereas other tumor suppressor genes showed very low methylation. Thirteen of 30 (43%) tumor-derived cell lines, and U-2OS and SaOS-2 showed hypermethylation of the RUNX3 gene on MSPCR. However, RUNX3 was expressed in the SaOS-2 cell line, as determined by RT-PCR, and the expression was augmented by treatment with 5-aza-2' deoxycytidine.CONCLUSION:
Our study suggests that aberrant methylation is an important mechanism of RUNX3 down-regulation in osteosarcoma. This data may have potential significance in developing a potential therapeutic target for osteosarcoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteosarcoma
/
Down-Regulation
/
Cell Line
/
Polymerase Chain Reaction
/
Genes, Tumor Suppressor
/
CpG Islands
/
Deoxycytidine
/
Methylation
Limits:
Humans
Language:
Korean
Journal:
The Journal of the Korean Orthopaedic Association
Year:
2009
Type:
Article
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