Minocycline Protects Vestibular Hair Cells from Neomycin-Induced Ototoxicity by Inhibiting Reactive Oxygen Species Production and Caspase-3 Activity / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 1159-1164, 2006.
Article
in Korean
| WPRIM
| ID: wpr-656560
ABSTRACT
BACKGROUND AND OBJECTIVES:
Aminoglycosides, a commonly used antibiotic agent, destroys the sensory hair cells in the cochlear and vestibular system leading to irreversible hearing loss and balance problem. Minocycline, a second-generation tetracycline antibiotic, has been known to possess anti-apoptotic properties in addition to its antimicrobial action. We hypothesized that minocycline would attenuate aminoglycosides induced vestibulotoxicity in rat utricles. SUBJECTS ANDMETHOD:
Utricular maculae prepared from postnatal day 3-4 rats were treated with neomycin alone or in combination with minocycline. For hair cell count, utricles were stained with phalloidin-FITC. Reactive oxygen species (ROS) was assessed using the fluorescent probe, hydrofluorescent diacetate acetyl ester (H2DCFDA). Caspase-3 activity was also examined using the fluorescent caspase-3 substrate.RESULTS:
Neomycin induced dose-dependent loss of utricular hair cells. Minocycline reduced ROS production and caspase-3 activation in neomycin treated utricular hair cells.CONCLUSION:
Minocycline has protective effect in neomycin induced ototoxicity in rat utricle by inhibiting ROS production and caspase-3 activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tetracycline
/
Neomycin
/
Cell Count
/
Saccule and Utricle
/
Reactive Oxygen Species
/
Hair Cells, Vestibular
/
Caspase 3
/
Aminoglycosides
/
Hair
/
Hearing Loss
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2006
Type:
Article
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