The Effect of Genotype of NAT2, TNF-alpha and TGF-beta Genes on Allergic Rhinitis in Korea / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: The inflammatory reaction is affected by the genotype or phenotype of genes related with inflammation modulating cytokines and allergen metabolizing enzyme. This study was performed to investigate the relationship between N-acetyl transferase 2 (NAT2), tumor necrosis factor-alpha(TNF-alpha), transforming growth factor-beta(TGF-beta) and the allergic rhinitis in Korea. SUBJECTS AND METHOD: One hundred forty five allergic rhinitis patients and 167 controls were included. Venous blood samples were collected and the genotypes were analyzed by PCR technique. NAT2 phenotypes were designated as 'high', 'medium' and 'low' by known genotype-phenotype relationship. TNF-alpha genotypes were classified as 'A/A', 'A/G' and 'G/G'. TGF-beta genotype was classified as 'Arg/Arg', 'Arg/Pro' and 'Pro/Pro'. These data were analyzed by SAS for windows ver 8.02. RESULTS: The phenotype of NAT2 and genotype of TGF-beta showed no significant effect on allergic rhinitis. G/G or G/A genotype of TNF-alpha significantly increases the risk of allergic rhinitis. High activity phenotype of NAT2 showed higher level of total serum IgE. CONCLUSION: Genetic factors including TGF-beta are important in the development of allergic rhinitis in Korea. NAT2 phenotype affect allergic reaction.