In situ hybridization Study on the Age-related Changes of Ghrelin mRNA Expression in Rat Stomach / 대한해부학회지
Korean Journal of Anatomy
;
: 167-173, 2003.
Article
in Korean
| WPRIM
| ID: wpr-656912
ABSTRACT
Ghrelin is a recently identified member of growth hormone secretagogue, which is synthesized as peptide of 28 amino acids in stomach, pituitary and intestines. Ghrelin controls food intake, and induces gastric acid secretion. It has been reported that synthesis and secretion of ghrelin changes according to age of animals. In this study, in situ hybridization technique with digoxigenin-labeled oligonucleotide probe is applied to elucidate the developmental change of ghrelin mRNA expression pattern in rat stomach. Ghrelin mRNA positive cells first appeared in stomach of 9 day rats, mainly in base of gastric glands. It showed the highest density and spread to neck area of the gastric gland in 6 week rats. Number of positive cells decreased significantly in 18 months. Most of ghrelin mRNA positive cells were found as closed-type endocrine cells in fundus area. These results suggest that ghrelin expression may be involved in development and ageing process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
RNA, Messenger
/
Growth Hormone
/
In Situ Hybridization
/
Eating
/
Ghrelin
/
Endocrine Cells
/
Gastric Acid
/
Gastric Mucosa
/
Amino Acids
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Anatomy
Year:
2003
Type:
Article
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