Distribution and typing of animal-derived Salmonella with MLST and serotype / 中国人兽共患病学报

ABSTRACT

To compare the discriminatory ability of multilocus sequence typing and serotype of animal-derived Salmonella and find its distribution in Shandong Province,78 chicken-origin,56 duck-origin and 20 swine-origin Salmonella were separated from some regions of Shandong Province.Seven conserve sequences of Salmonella were PCR-amplified for MLST and slide agglutination test for serotyping.Results showed that by serotyping,6 serotypes were identified from chicken-origin Salmonella,including 88.5％0 S.enteritidis,5.1％ S.indiana,2.6％ S.thompson,1.3％ S.typhimurium,1.3％ S.senftenberg,1.3％ S.agama.Two serotypes were identified from duck-origin Salmonella,including 67.9％ S.enteritidis,32.1％ S.ty phimurium.Three serotypes were identified from swine-origin Salmonella,including 65％ S.typhimurium,20％ S.derby,and 15％ S.enteritidis.By MLST typing,seven ST types were identified from chicken-origin SalmonellaST11,ST19,ST26,ST128,ST14,ST17 and Newl.Three ST types were identified form duck-origin SalmonellaST11,ST19 and New2.Three ST types were identified from swine-origin SalmonellaST34,ST40 and ST3007.Overall,the types identified with two methods were closed,so MLST and serotype have similar discriminatory ability.