Effects of Rule Granules on Breast Tissue, Sex Hormones and Receptor Expression of Mammary Glands Hyperplasia Rats / 中国中医药信息杂志

ABSTRACT

Objective To observe the effect of Rule Granules on the breast tissue pathology,ovarian hormone and receptor expression of mammary glands hyperplasia rats; To explore its mechanism of action. Methods The model of mammary gland hyperplasia was established by intramuscular injection of estradiol benzoate for 20 days and intramuscular injection of progesterone for 5 d. 66 female SD rats were randomly divided into 6 groups, including blank group, model group, acupuncture group, TCM high-, medium- and low-dose groups, with 11 rats in each group. Acupuncture group chose group A (Wuyi, Hegu, on both sides, Tanzhong) and group B (Tianzong, Ganshu, Zusanli, on both sides), and the two groups were used alternately, one group per day. TCM groups received 1.1 g/mL, 0.55 g/mL,and 0.11 g/mL dose of Rule Granules for gavage,once a day.Abdominal aorta blood was collected after continuous treatment of 30 d. The contents of serum E2and P were detected through ELISA method, at the same time, the second left side of the breast was removed. The changes of the breast tissue was observed under light microscope, and the ER and PR positive expression was observed by SABC immunohistochemical method. Results Compared with the blank group, hyperplasia of mammary gland in the model group rats was more obvious, and serum E2, ER and PR positive expression of integral increased significantly (P<0.01), but the content of P decreased significantly (P<0.01); compared with the model group, hyperplasia of mammary gland was improved in all treatment groups, and serum E2, ER and PR positive expression of integral decreased significantly (P<0.05); the content of P in acupuncture group increased significantly (P<0.01), and the acupunture group and TCM low-dose group were the most obvious. Conclusion Rule Granules can effectively improve hyperplasia of mammary gland organization structure in rats caused by exogenous estrogen and progesterone, with TCM low-dose group and acupuncture group having the best efficacy. Its mechanism is related to reducing the contents of serum E2and ER, and PR positive expression in breast tissue, and increasing the content of serum P.