Experimental Study on the Effect of Qingfei Decoction in Antifibrosis in Rats with Connective Tissue Disease Related Interstitial Pneumonia / 浙江中医药大学学报

ABSTRACT

[Objective]To establish a rat model of connective tissue disease associated interstitial pneumonia induced by bleomycin, and explore the effect of Qingfei Decoction on interstitial pneumonia and its effect on antifibrosis. [Methods]48 male SD rats , weight 180~200g. All the rats were fed adaptivelly for one week and the rat model of interstitial lung disease were established by means of pour 5mg·kg-1 BLM into trachea .Divided these rats into 4 groupsthe normal control group, the interstitial pneumonia model group ,the predmisone group and the Qingfei Decoction group. In addition, each group 12 rats. After 28 days'medication, compare the pathological difference between Qingfei Decoction group with the interstitial pneumonia model group, the predmisone group and the normal control group, and the difference in the expressions of TNF-α, IL-6, TGF-β1 in lung tissue, and the expression of hydroxyproline in lung tissue. [Result]①Pathological staining showed thatcompared with the normal control group, the destruction of lung tissue structure of rats pneumonia, severe inflammatory cell infiltration, fibrous tissue hyperplasia massive consolidation, alveolar distribution of collagen fiber lesions in model groups; compared with interstitial pneumonia model group, Qingfei Decoction group reduced lung tissue damage, reduce inflammation cell infiltration, fibrous tissue hyperplasia reduced collagen relative area decreased compared with model group. ②Compared with normal control group, the lung tissue of HYP rats was significantly increased, and the difference was statistically significant the model group( P<0.01); Compared with interstitial pneumonia in the model group, the content of HYP in lung tissue of rats Qingfei Decoction group decreased significantly, and the difference was statistically significant(P<0.01). ③Compared with normal control group, the model group of rat lung tissue IL-6, TNF- alpha, TGF- beta1 concentration increased significantly(P<0.05, P<0.01);compared with the model group, interstitial pneumonia, Qingfeifang lung tissue homogenate TNF-alpha, TGF-beta 1, IL-6 concentration decreased significantly(P<0.05, P<0.01). [Conclusion]1.Qingfei Decoction has inhibition effect to the bleomycin induced interstitial pneumonia.2.The treatment mechanism of Qingfei Decoction to BLM-ILD maybe related to TNF-α, IL-6, TGF-β1.