The application design of carbon nanoparticle immunomarker chromatography in fecal occult blood, calcium and cancer embryo antigen / 中国生化药物杂志

ABSTRACT

Objective To design a method for rapid detection of fecal occult blood, calcium and cancer embryo antigen based on the immunochromatography of nanoparticles. Methods Used nano carbon lateral flow immune chromatography technology which applicated in trace detection of drug development,choose human hemoglobin,calcium,protein,carcinoembryonic antigen suitable double antibody. 1 resistance of three test taged by colloidal carbon probe cured in carbon mat, and the corresponding test pairs of another plant 2 fixed on nitrocellulose membrane resistance test region(Line T), quality control line(Line C) which composed of sheep anti-mouse IgG antibody,glue water cushion,nitrocellulose membrane,colloidal carbon release pad and sample pad, drying cutting made test card.Choose 100 cases of endoscopic check negatie and 100 cases of colorectal cancer patients with intestinal preparation before stool specimens, dropped the dissolved the centrifugal supernatant in normal saline into the strip and put the special card slot, applied independent research of real-time quantitative detector checked the content of fecal occult blood, the calcium content of protein, carcinoembryonic antigen respectively. Results The application of nano carbon lateral flow immune chromatography test card for fecal occult blood calcium, protein, carcinoembryonic antigen was conveniented.Real-time quantitative detector check readable data range of 0-7000, the sensitivity data of 0.2 ng/mL. The positive rate of endoscopic check negatie and colorectal cancer patients with fecal occult blood were 1.91% and 77.01% respectively,the average content of calprotectin were (26.81±5.96) μg/g and (589.00±42.29) μg/g. Average content of carcinoembryonic antigen were (1.21±0.45) μg/g and (234.32±11.24) μg/g respectively. Conclusion The rapid detection method of nanometer carbon particle immunochromatography was reliable, and fecal occult blood, calcium protamine and cancer embryo antigen jiont test was value for colorectal cancer screening.