Effects of miR-210 on Mex3 B protein on migration and proliferation of lung cancer cells / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of miR-210 on the migration and proliferation of lung cancer cells. Methods:The expression of miR-210 in normal lung tissues and lung cancer tissues were detected by qPCR. The expression of Mex3B in lung cancer tissues and adjacent tissues were detected by qPCR. qPCR was used to detect the expression of miR-210 in different lung cancer cell lines(A549,H1299,H1650 and H358). The effect of miR-210 on the transcription of Mex3B was detected by double luciferase reporter gene system. The effect of miR-210 expression on the activity of lung cancer cells was detected by cell viability assay. The effect of miR-210 on the proliferation of A549 cells were detected by plate cloning assay. Transwell invasion assay were used to detect the effect of miR-210 on the invasion ability of lung cancer cell line A549. Results:Compared with adjacent tissues,the expression of miR-210 were significantly decreased in lung cancer tissues,Mex3B were lower in lung cancer. Double luciferase reporter gene system results showed that miR-210 can directly regulate the transcriptional activity of Mex3B. The invasion and proliferation ability of lung cancer cell line were significantly reduced after inhibition of miR-210. Conclusion:miR-210 can target regulate the expression of Mex3B, and affects the invasion and proliferation of lung cancer cells.