Clinicopathological significance of Musashi 2 expression in human colorectal cancer / 中华普通外科杂志

ABSTRACT

Objective To study the clinicopathological signiticance of the expression of musashi2 (MSI 2) protein and mRNA levels in human colorectal cancer (CRC).Methods The expression of MSI 2 protein in 85 CRC specimens and paired adjacent non-cancerous tissues were detected by immunohistochemistry (IHC).The relationship between the protein expression and clinicopatho]ogical features was analyzed.Immunoblotting and real time quantitative PCR were used to examine the expression of MSI 2 protein and mRNA levels in 12 paired fresh CRC and adjuvant non-cancerous tissues.Results MSI 2 overexpression was found in 45 cases of 75 CRC tissues,which was much higher than that in noncancerous tissues (59％ vs.30％,P ＜ 0.01).MSI 2 overexpression had a positive correlation with tumor size (x2 =7.682,P =0.006),T stage (x2 =4.218,P =0.040),Dukes stage (x2 =8.590,P =0.014),and Ki67 expression (x2 =6.412,P =0.011).Moreover,CRC patients with MSI 2 overexpression had a worse prognosis (x2 =4.855,P =0.028).Both MSI 2 protein and mRNA levels in 12 cases of CRC tissues were much higher than that in non-cancerous tissues (t =3.323,P ＜ 0.01;t =2.673,P =0.022,respectively).Conclusion MSI 2 overexpression is closely related with tumor size,T stage,Dukes stage,Ki67 expression and poor prognosis of CRC patients.