Breeding and genotyping of a miR-5572transgenic mouse model of sick sinus syndrome / 中国实验动物学报
Acta Laboratorium Animalis Scientia Sinica
;
(6): 467-472, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-658030
ABSTRACT
Objective This study aimed to explore the possibility of establishing a model of sick sinus syndrome by using miR-5572 transgenic mice. Methods F1 and F2 miR-5572 transgenic mice were bred and genotyped, and then observed the phenotype levels of miR-5572 transgenic mice by morphology, electrocardiogram record ( ECG ) and the Cav1. 2 and Cav1. 3 expressions levels of mRNA and protein in sinoatrial node tissue of homozygous, heterozygote and wild type mice. Results Compared with the wild type and heterozygous mice,the miR-5572 homozygous mice showed a devel-opment delay and smaller body shape,and had slower average heart rate. The mRNA and protein levels of Cav1. 2 and Cav1. 3 in the sinoatrial node tissues were significantly lower. Conclusions The results of this study indicate that miR-5572 homozygous mice may be an efficient approach to establish the model of sick sinus syndrome
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Laboratorium Animalis Scientia Sinica
Year:
2017
Type:
Article
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