Simultaneous Determination of Six Components in Huoxiang Zhengqi Series Preparations by HPLC / 中国药师

ABSTRACT

Objective:To establish an HPLC method for the determination of hesperidin, imperatorin, isoimperatorin, magnolol, honokiol and atractylodin in Huoxiang Zhengqi series preparations. Methods:An HPLC method was used with a Waters C18 chromato-graphic column (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of acetonitrile-0. 5% glacial acetic acid aqueous solution re-spectively for the mobile phase A and B with gradient elution. The flow rate was 1. 0ml·min-1;the detection wavelength was 290 nm;the column temperature was set at 40℃;the sample size was 10μl. Results:The linearity ranges of hesperdin, imperatorin, isoimper-atorin, magnolol, honokiol and atractylodin showed good linear relationships(r≥0.9993). The average recovery was 97.0%, 95. 0%,94. 3%,97. 6%,97. 3% and 93. 2%, respectively. The RSD was 2. 25%,2. 60%,2. 51%,1. 67%,1. 78% and 2. 50%( n=9), respectively. Conclusion:The method is simple and quick with good reproducibility, high precision and promising applicabili-ty, which can be used for the quality control of Huoxiang Zhengqi series preparations.