Effects of chemokine Fractalkine on proliferation and invasion of human pancreatic cancer cell lines by regulating IL-6/STAT3 signal pathway / 重庆医学
Chongqing Medicine
;
(36): 3893-3895,3899, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-658585
ABSTRACT
Objective To explore the effects of chemokine Fractalkine(FKN) on the proliferation and invasion of human pancreatic cancer cell lines PANC-1 and SW-1990 by regulating IL-6/STAT3 signal pathway.Methods Adenovirus served as the vector to construct and synthesizing FKN-small interfering RNA(siRNA),then which was transfected into PANC-1 and SW-1990.The proliferation and invasion ability of cells was determined by CCK-8 assay and Transwell assay.Expression of FKN,IL-6 and STAT3 protein and mRNA was detected by Western blot and RT-qPCR.Results After transfecting FKN-siRNA for 24 h,the absorbance values(A value) in the PANC-1 and SW-1990 groups had no significant changes,the A value at 48,72 h in the FKN-siRNA group was significantly higher than that in the control group and FKN-siRNA negative group (P<0.05).After transfecting FKN-siRNA,the cellular invasive ability in the PANC-1 and SW-1990 FKN-siRNA group was significantly stronger than that in the control group and FKN-siRNA negative group(P<0.05).After transfecting FKN-siRNA in cell lines PANC-1 and SW-1990,compared with the control group and FKN-siRNA negative group,the FKN protein and mRNA expression in the FKN-siRNA group was significantly decreased(P<0.05),while IL-6 and STAT3 protein and mRNA expression was significantly increased(P<0.05).Conclusion Chemokine FKN might play the inhibiting effect on the biological activity of pancreatic cancer cells by regulating IL-6/ STAT3 signal pathway.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chongqing Medicine
Year:
2017
Type:
Article
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