The Cytology for Leukemic Cells in Cerebrospinal Fluid; Comparison of Conventional Cytology with Liquid-Based Cytology
Korean Journal of Pathology
;
: 164-170, 2009.
Article
in Korean
| WPRIM
| ID: wpr-65900
ABSTRACT
BACKGROUND:
The cytological examination of cerebrospinal fluid (CSF) using conventional cytology with a cytocentrifuge (cytospin) is an important method for evaluating the involvement of leukemia in the CNS. Liquid-based cytology (LBC) is now a widely used cytological method not only for gynecological and non-gynecological specimens, but its application to CSF for the identification of leukemic cell has not yet been reported. In this study, we tried to compare conventional cytology with using a cytospin with LBC and Papanicolaou (Pap) staining. We also examined the modified LBC with Wright staining to assess whether this modified method can be useful for diagnosing Leukemia.METHODS:
We studied 30 cases of CSF that were obtained from 16 patients, including 17 cases of acute myeloid leukemia, 12 cases of acute lymphoblastic leukemia and 1 case of diffuse large B cell lymphoma. We applied conventional cytology with a cytocentrifuge (cytospin), LBC with Pap staining and modified LBC with Wright staining.RESULTS:
The morphological features of the LBC with Pap staining showed difficulty for interpretation when compared with conventional cytology with a cytospin, and mainly because of cellular shrinkage. The modified LBC with Wright staining showed good morphological features.CONCLUSIONS:
We suggest that modified LBC with Wright staining may be useful for examining CSF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukemia, Myeloid, Acute
/
Leukemia
/
Lymphoma, B-Cell
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2009
Type:
Article
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