Methylation status of TES gene promoter region in 25 patients with leukemia / 国际检验医学杂志

ABSTRACT

Objective To study the methylation status of Testin protein (TES) promoter region in bone marrow of leukemia pa-tients ,and to explore the relationship between TES gene and leukemia .Methods The bone marrow specimens of 13 patients with primary acute myeloid leukemia and 12 patients with acute myeloid leukemia were studied .Bone marrow samples from 15 healthy subjects were used as controls ,the methylation level of TES gene promoter in bone marrow was detected by BSP ,the methylation status between healthy subjects and leukemia patients ,and whether there was any difference in methylation status between different types of leukemia were analyzed .Results In 25 cases of leukemia ,there were 21 cases of bone marrow samples ,and the hypermeth-ylation of TES gene promoter region was positive ,with a positive rate of 84 .0% .The methylation of the gene was not detected in bone marrow samples from 15 healthy subjects .The methylation rate of TES gene in bone marrow samples of 12 patients with acute lymphoblastic leukemia was 83 .3% ,the methylation rate of TES gene in bone marrow samples of 13 patients with primary acute myeloid leukemia was 84 .6% ,and there was no significant difference between the two groups (P>0 .05) .Conclusion Methylation of TES gene might be one of the biomarkers in the diagnosis of leukemia .