Influence of glycyrrhizic acid on oxidative stress injury of glomerular mesangial cells induced by high glucose / 重庆医学

ABSTRACT

Objective To investigate the influence of glycyrrhizic acid (GA) on oxidative stress injury in glomerular mesangial cells (HBZY-1) induced high glucose.Methods HBZY-1 was cultured and divided into the normal control group,high glucose group and high glucose +-GA group.The cell proliferation activity was measured by MTT assay.UV spectrophotometry was used to detect the SOD and MDA levels.And the ROS changes were detected by laser confocal microscopy.Immunohistochemistry and Western blot were used to detect the expression of Mn-SOD protein in various groups.Mn-SOD mRNA was detected by Q-PCR.Results (1) The morphological changes in each groupHBZY-1 cells were diamond-shaped,cells were normal and the structure was clear in the NG group.In the HG+-GA group,the number of cells was increased slightly,individual cell body was slightly hypertrophy.The cell structure in the HG group was not clear,the cells appeared flat,the number was increased significantly,the cell body was slightly hypertrophy.(2)The proliferative effect of HBZY-1 in each groupcompared with the NG group,the OD value in the HG group was significantly increased,and the OD value in the HG+GA group was decreased compared with the HG group(P＜0.05).(3)The relative content of ROS in the HG group was higher than that in the NG group,and which in the HG+GA group was decreased (P＜0.05).(4)The expression of Mn-SOD in each groupthe relative expression of Mn-SOD in the HG group was significantly lower than that in the NG group (P＜0.05),and the expression of Mn-SOD in the HG+-GA group was increased compared with the HG group(P＜0.05).Conclusion Glycyrrhizic acid has a certain inhibiting effect on the abnormal proliferation of HBZY-1 induced by high glucose.Glycyrrhizic acid can protect the cell hypertrophy and cell damage caused by glomerular mesangial cells via oxidative stress,and glycyrrhizic acid can regulate the Mn-SOD expression induced by high glucose.