Effect of Sevoflurane Preconditioning on Lung Injury Induced by Ischemia/reperfusion of Hind Limbs in Rats / 医学研究杂志

ABSTRACT

Objective To investigate the effect of sevoflurane (Sevo) preconditioning on lung injury induced by ischemia/reperfusion (IR) of hind limbs in rats.Methods Forty-five healthy adult Sprague-Dawley (SD) rats of clean grade were randomly allocated into three groups,15 mice per group.Femoral veins and femoral arteries of rats were isolated but not clipped in Sham operation group (Sham group).Femoral arteries of rats were clipped for ischemia for 3h,followed reperfusion for 3h in ischemia/reperfusion group (IR group).Rats were inhaled with 2.5％ sevoflurane for 30 min,followed establishing the model of IR of hind limbs after 15min in Sevo preconditioning + IR group (SIR group).Rats were euthanized after experimental time out,and left lung tissue was excised.Wet lung weight to dry lung weight (W/D) and total lung water content (TLW) were tested.Pathological changes of lung tissue were observed and index of quantitative evaluation for alveolar damage (IQA) was tested by light microscope,and changes of ultrastructure of lung tissue were observed by transmission electron microscope.The expressions of glucose-regulated protein 78 (GRP78) and CCAAT enhancer binding protein (C/EBP) homologous proteins (CHOP) protein were detected by reverse transcription PCR (RT-PCR) and Western Blot,respectively.Results Compared to Sham group,W/D,TLW and IQA were all notably higher (P ＜ 0.05) in IR group.W/D,TLW and IQA of SIR group were all lower (P ＜ O.05) than those of IR group.The injury of morphological structure and ultrastructure of lung tissue was distinct in IR group but alleviated in SIR group.Compared to Sham group,the expression of GRP78 and CHOP was higher (P ＜ 0.05) in IR group.The expression of GRP78 and CHOP in SIR group was lower (P ＜ 0.05) than that in IR group.Conclusion Sevo preconditioning has protective effect on rats with lung injury induced by IR of hind limbs,which may be related to inhibition of excessive unfolded protein response (UPR).