Expression of p66Shc in heart failure patients and its relationship with oxidative stress / 中国综合临床

ABSTRACT

Objective To investigate the expression of cardiac muscle protein p66Shc in patients with congestive heart failure and its relationship with oxidative stress.Methods From October 2016 to March 2017, eighty patients with acute heart failure treated in the cardiology department of Shanghai Pudong Hospital were collected in this study,including 22 cases of grade II,26 cases of grade III,32 cases of grade IV,and 56 healthy subjects were selected in the control group.SOD activity,MDA content and p66Shc mRNA expression of the acute stage of heart failure,the recovery stage of heart failure and the control group were measured.The changes of SOD,MDA and p66Shc in patients with heart failure and the control group were analyzed.The expression of p66Shc,along with MDA and SOD were analyzed.Results There were significant differences in the SOD,MDA and p66Shc mRNA of patients in the acute stage of heart failure,the recovery stage and the control group were (SOD(65.67±8.31)kU/L vs.(75.77±7.56)kU/L vs.(90.03±7.76)kU/L,MDA(10.22±1.56)μmol/L vs.(8.34 ± 1.75)μmol/L vs.(5.21 ± 2.16)μmol/L,p66Shc mRNA(5.79 ± 1.71)vs.(3.95 ± 1.24) vs.1.87±0.93))(F=2.903,2.762,2.696,P<0.05).With the aggravation of heart failure,the level of SOD decreased significantly(NYHA grade Ⅱ(67.54 ± 7.29)kU/L;grade Ⅲ(61.62 ± 6.96)kU/L;gradeⅣ(57.94±6.75)kU/L,F=2.872,P<0.05)and the levels of MDA(NYHA grade Ⅱ(9.32±1.18)μmol/L;grade Ⅲ(10.95 ± 1.26)μmol/L;grade Ⅳ(12.13 ± 1.07)μmol/L,F=2.703,P<0.05)and p66Shc (NYHA grade Ⅱ(4.92 ± 0.96);grade Ⅲ(5.66 ± 1.17);grade Ⅳ(6.28 ± 1.15),F=2.681,P<0.05) increased significantly.The expression of p66Shc and mRNA was positively correlated with MDA in acute attack (r=0.722,P<0.05),and negatively correlated with SOD(r=-0.670,P<0.05).Conclusion P66shc is closely related to oxidative stress in heart failure,and p66Shc may be involved in the process of heart failure oxidative stress,which can provide a basis for the new treatment of heart failure.