Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats
Journal of Periodontal & Implant Science
;
: 244-253, 2016.
Article
in English
| WPRIM
| ID: wpr-65954
ABSTRACT
PURPOSE:
The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy.METHODS:
Twenty-eight male Sprague-Dawley rats (body weight, 250–300 g) were randomly divided into two treatment groups with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis.RESULTS:
Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found.CONCLUSIONS:
Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Parietal Bone
/
Calcium
/
Rats, Sprague-Dawley
/
Bone Substitutes
/
Transplants
/
Hyperbaric Oxygenation
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Journal of Periodontal & Implant Science
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS