Experimental Analysis of SD Rat Model of Pre-eclampsia by Using Two Different Modeling Methods / 实用妇产科杂志

ABSTRACT

Objective:This study was aimed to compare the advantages and specifity of two different models to construct the animal model of preeclampsia in prague-Dawley(SD) rats.Methods:Female pregnancy SD rats were randomly divided into three groups (n =30 per group)Control group,STBM group and L-NAME + STBM group.The model of preeclampsia(PE) was established via intravenous or subcutaneous injection of STBM or L-NAME + STBM.In the STBM group,STBM were injected via the vena caudalis.In the L-NAME + STBM group,Nw-nitro-L-arginine-methyl ester(L-NAME) was injected subcutaneously.Control group got no treatment.Proteinuria(U-TP),blood pressure(BP),coagulation parameters (APTT,PT,and D-D) were tested at different times.Placental sections were histopathologically examined by H-E stanning.SFIt-1,SEng,PLGF mRNA and protein expression in placenta were tested by Real time PCR and Western blot respectively.Placental trophoblast cells were isolated and cultured,for advanced functional experiment.Results:There was no significant difference in U-TP,BP,APTT,PT and D-D between the 3 groups on pregnant 10D(P＞0.05),when compared with STBM group,U-TP,APTT,PT and D-D were significantly increased in STBM + L-NAME group on pregnant 15 d and 20 d (P ＜0.05),and there was no significant change in control group.The expression of SFlt-1,SEng mRNA and protein were significantly different between the 3 groups (P ＜ 0.05).The expression of sFIt-1,SEng mRNA and protein were significantly higher in STBM + L-NAME groupthan that in STBM group(P ＜ 0.05),with sigficantly reduced PLGF mRNA and protein in STBM + L-NAME group(P ＜0.05).There was a significant difference between the 3 groups in functional experiment(P＜0.05).The cell proliferation and invasion rate was lowest and the apoptosis rate was highest,with significant difference when compared to those in STBM group(P ＜ 0.05).Conclusions:STBM combined with L-NAME was more specific than STBM alone,to construct the model of preeclampsia in SD rats and provide a reliable basis for clinical diagnosis and treatment of preeclampsia.