Simultaneous determination of seven constituents in Dangshen Guben Pills by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martynoside,lobetyolin,methylophiopogonanone A and methylophiopogonanone B in Dangshen Guben Pills (Rehmanniae Radix,Rehmanniae Radix Preparata,Codonopsis Radix,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 30 ℃C thermostatic Agilent TC-C1s column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,330,269 and 296 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 2),whose average recoveries were 96.96％-99.65％ with the RSDs of 0.88％-1.74％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dangshen Guben Pills.