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Simultaneous Quantitative Determination of Five Saponins in Notoginseng Total Saponins by HPLC with a Monolithic Column / 中国药师
China Pharmacist ; (12): 1879-1881, 2017.
Article in Chinese | WPRIM | ID: wpr-660865
ABSTRACT

Objective:

To establish a rapid HPLC method for the quantitative determination of 5 saponins ( notoginsenoside R1 , ginsenoside Rg1 ,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd) in Notoginseng total saponins using a monolithic column. Meth-odsThe analysis was performed on a Merck Chromolith Performance RP-18e column (100 mm × 4. 6 mm,2μm) with gradient elution of acetonitrile and water. The detection wavelength was set at 203 nm.

Results:

Satisfactory separation of all analytes was obtained in 20 min. All calibration curves showed good linearity within the testing ranges (r≥0.9998). The average recoveries were between 98. 6% and 100. 4%. The RSDs were less than 2. 1% (n=6).

Conclusion:

The method is efficient and accurate for the quality con-trol of Notoginseng total saponins.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article