Simultaneous Determination of 6 Components in Niuhuang Ninggong Tablets by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for simultaneous determination of 6 components in Niuhuang ninggong tablets.METHODS:HPLC method was adopted.The determination was performed on TC-C18 column with mobile phase consisted of methanol-0.05％ phosphate acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 280 nm,and the column temperature was 25 ℃.The sample size was 10 μL.RESULTS:The linear ranges of glycyrrhizin,berberine hydrochloride,baicalin,baicalin,emodin and chrysophanol were 3.2-320 μg/mL(r=0.999 9),8.8-880 μg/mL(r=0.999 8),5.6-560 μg/mL (r=0.999 5),2.0-200 μg/mL(r=0.999 9),4.4-440 μg/mL (r=0.999 9),2.0-200 μg/mL(r=0.999 7),respectively.RSDs of precision,stability and reproducibility tests were all lower than 6.0％.The recoveries were 96.34％-97.25％ (RSD=0.33％,n=6),96.12％-98.06％ (RSD=0.82％,n=6),96.36％-99.09％ (RSD=1.02％,n=6),95.84％-97.32％ (RSD=0.65％,n=6),95.83％-97.92％(RSD=0.88％,n=6),98.60％-99.65％(RSD=0.42％,n=6),respectively.CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for content determination of 6 components in Niuhuang ninggong tablets.