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Comparison of Protein Content Determination Respectively by Folin-Ciocalteu Method and Coomassie Bril-liant Blue Binding Method for Mannatide Oral Solution / 中国药师
China Pharmacist ; (12): 1861-1863, 2017.
Article in Chinese | WPRIM | ID: wpr-661098
ABSTRACT

Objective:

To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results.

Methods:

The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sultsAs for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method.

Conclusion:

Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article