Isolation and identification of exosomes of CD4 +T cells in mice / 中国比较医学杂志

ABSTRACT

Objective To explore whether CD4 +T cells in mice can secrete exosomes and the possibility of exosomes to participate in the inflammation and immune response process. Methods Spleen samples were taken from mice, and CD4 +T cells were isolated from the spleen tissue using magnetic bead separation method. Exosomes of CD4 + T cells were extracted from the supernatant and observed under transmission electron microscope. PCR assay was used to detect the expression of miR-23a and miR-214. Results Exosomes were observed under elecron microscope, and the expression of miR-23a and miR-214 were detected by PCR assay. Conclusions CD4 + T cells in mice can secrete exosomes,which offer possibility to participate in the inflammation and immune response.