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Simultaneous determination of five constituents in Shanzhuyu Formulated Granules by QAMS / 中成药
Chinese Traditional Patent Medicine ; (12): 1845-1849, 2017.
Article in Zh | WPRIM | ID: wpr-661669
Responsible library: WPRO
ABSTRACT
AIM To establish a quantitative analysis of multi-components by single mark (QAMS) method for the simultaneous content determination of five constituents in Shanzhuyu Formulated Granules (Corni Fructus).METHODS The analysis of 80% methanol extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.3% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 335 nm.With morroniside as an internal standard,the relative correction factors of gallic acid,5-hydroxymethylfurfural,loganin and cornuside were established,followed by the determination of their contents.RESULTS Gallic acid,5-hydroxymethylfurfural,morroniside,loganin and cornuside showed good linear relationships within the ranges of 0.0120-0.120,0.026 8-0.268,0.074 4-0.744,0.058 6-0.586 and 0.0086-0.086 μg (r≥ 0.9999),whose average recoveries (RSDs) were 103.43% (1.45%),103.36% (1.50%),104.47% (0.30%),102.08%(1.74%) and 104.01% (0.62%),respectively.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This stable and reliable method can be used for the quality control of Shanzhuyu Formulated Granules.
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Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article