Mechanism of berberine-induced apoptosis in cervical cancer HeLa cell line / 基础医学与临床

ABSTRACT

Objective To study the apoptosis mechanism of HeLa cells induced by berberine (BR). Methods HeLa cells were treated by different concentrations of BR with different times. CCK-8 assay was used to detect the HeLa cells proliferation activity. Detecting the expression of STAT3 in control HeLa cells and BR treated HeLa cells. HeLa cell cycles were detected by flow cytometry(FCM). The relative expression of STAT3, CYCLIN B1, CDC2 and C-MYC was examined by real-time PCR. The relative expressions of STAT3, CYCLIN B1, CDC2 and C-MYC proteins were examined by Western blot analysis. Results BR can effectively inhibit the proliferation of HeLa cells in vitro, which exhibits a dose-dependent and time-dependent manner. The FCM showed that the the proportion of BR-treated cells in G2/M phase was higher than control group. Real-time PCR results showed that rel-ative expression of STAT3,CYCLIN B1,CDC2 and C-MYC genes in HeLa cells treated with BR was lower than the control group.Western blot results showed that relative expression of STAT3,CYCLIN B1,CDC 2 and C-MYC pro-teins in HeLa cells treated with BR was lower than the control group. Conclusions BR can induce apoptosis of cer-vical cancer by targeting STAT3.