Simultaneous Determination of Chlorogenic Acid, Baicalin and Quercetin in Compound Yuxingcao Tablets by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.METHODS:HPLC method was adopted.The separation was performed on GL InterSustain-C18 column with mobile phase consisted of water-methanol (55∶45,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm,and the column temperature was 30 ℃.The sample size was 20 μL.RESULTS:Linear ranges of chlorogenic acid,baicalin and quercetin were 1.264-12.64 μg/mL (r=0.999 9),8.704-87.04 μg/mL(r=0.999 9) and 1.212-12.12 μg/mL(r=0.999 9),respectively.The limit of quantification were 1.88,2.98,1.94 ng,respectively.The limits of detection were 0.56,0.89,0.58 ng,respectively.RSDs of precision,stability and reproducibility tests were all lower than 2.0％.The recoveries were 98.02％-101.10％ (RSD=1.01％,n=9),97.48 ％-101.70％ (RSD=1.38％,n=9) and 98.31％-101.10％ (RSD=1.02 ％,n=9),respectively.CONCLUSIONS:The established method is simple,accurate,stable and reproducible,and can be used for simultaneous determination of chlorogenic acid,baicalin and quercetin in Compound yuxingcao tablets.