Influence of puerarin on pancreatic tissue let-7 f and immune factors IL-17 and IL-23 expression of rats with gestational diabetes mellitus / 中国免疫学杂志

ABSTRACT

Objective:To explore the influence of puerarin on pancreatic tissue let-7f and immune factors IL-17 and IL-23 expression of rats with gestational diabetes mellitus .Methods:120 cases of rats were divided into the normal control group ,the blank control group,simvastatin group,puerarin low-dose group,puerarin medium-dose group and puerarin high-dose group.The normal control group were given basal diet,and the other groups were given high fat diet ,all fed for two weeks.The simvastatin group were given 2 mg/kg simvastatin,and the puerarin group were given 70 mg/kg,120 mg/kg,170 mg/kg puerarin according to low ,medium,high dose,and the normal control group and blank control group were given the same amount of normal saline ,after 2 weeks of treatment , blood sugar and blood lipid and let-7f expression in pancreatic tissue of rats were tested in the six groups .Results:After treatment, FBG,TC,TG and FFA of rats in the simvastatin group and puerarin group were significantly lower than those in the blank control group , and with the increasing of puerarin dose ,the levels decreased significantly (P<0.05),in a dose-dependent manner.Let-7f expression in pancreatic tissue of the simvastatin group and puerarin group was significantly higher than that in the blank control group (P<0.05),in a dose-dependent manner .IL-17 and IL-23 expression in serum and pancreatic tissue of the simvastatin group and puerarin group was significantly higher than that of the blank control group ( P<0.05 ) ,in a dose-dependent manner .Conclusion:Puerarin can increase let-7f expression in pancreatic tissue of gestational diabetes mellitus rats with gestational diabetes mellitus ,and can reduce IL-17 and IL-23 expression in serum and pancreatic tissue .It can provide reference for clinical treatment of gestational diabetes mellitus .