The research of Jianpi-Huatan decoction resisting MFC hepatic metastasis and its mechanism / 国际中医中药杂志

ABSTRACT

Objective To explore the research of Jianpi-Huatan decoction resisting the nude mouse MFC hepatic metastasis and its mechanism. Methods MFC cells inoculation in nude mice spleen, establishing nude mice hepatic metastasis model, which are divided into model group, 5-fu injection group, Jianpi-Huatan decoction high, medium and low dose group according to radom number table. Mice in high, medium and low dose Jianpi-Huatan decoction groups were adiminstrated with 80,40 and 20 g/kg Jianpi-Huatan decoction,in 5-fu groups were adiminstrated by intraperitoneal injection with 60mg/kg 5-fu injection and in model groups with Physiological saline once a day for 4 consecutive weeks. After the end, calculate the nude mice weight, spleen tumor weight and evaluation of hepatic metastases. And immune histochemical method and RT-PCR method is applied to detect tumor tissue of the expression of P53, Bcl-2 protein and mRNA. Results Compared with model group, Jianpi-Huatan decoction high and medium dose group can obviously increase body weight[(21.40 ± 1.43)g, (21.70 ± 1.02)g vs.(20.37 ± 1.17)g] and reduce the in situ tumor weight [(0.26 ±0.13)g,(0.16 ±0.05)g vs.(0.63 ±0.17)g]and the number of liver metastases;the mRNA levels of P53 and Bcl-2 (8.32 ±0.38,5.42 ±0.45,3.09 ±0.26 vs.9.67 ±1.31)and(4.65 ±0.61,3.22 ±0.21,2.49 ±0.19 vs.5.32 ±0.42) were decreased in low,medium and high dose Jianpi-Huatan decoction groups;P53(76.11 ±5.23,45.20 ±3.77, 23.11 ± 3.14 vs.81.63 ± 5.01)and Bcl-2(58.67 ± 5.27,32.00 ± 3.13,19.00 ± 2.54 vs.63.00 ± 4.10)levels were down-regulated in each dose of Jianpi-Huatan decoction group.Conclusions Jianpi-Huatan decoction can restrain nude mouse transplantation tumor growth and hepatic metastasis, which related to the cut of the expression of P53, Bcl-2 gene and protein.