Expression and clinical significance of p16 ,Ki-67 and CK7 in cervical precancerous lesion / 国际检验医学杂志

ABSTRACT

Objective To investigate the expression and clinical significance of p16 protein(p16) ,Ki-67 antigen(Ki-67) and cytokeratin 7(CK7) in cervical precancerous lesion .Methods The paraffin embedded tissue samples in 96 cases of cervical intraepithelial neoplasia(CIN) from January 2014 to March 2017 were selected and divided into the CIN Ⅰgroup(25 cases) ,CIN Ⅱgroup (36 cases) and CIN Ⅲ group(35 cases) according to the lesion severity ;meanwhile the paraffin embedded tissue samples in 64 ca-ses of chronic cervicitis were selected as the control group .The positive expression rates and expression modes of p16 ,Ki-67 and CK7 in different degrees of cervical lesion tissue were detected by adopting the immunohistochemistry method .Results The positive expression rates of p16 ,Ki-67 and CK7 in the CINⅠ ,Ⅱand Ⅲgroups were significantly higher than those in the control group , the difference was statistically significant (P＜0 .05) .The total score of p16 ,Ki-67 and CK7 had consistency with the degree of cervical precancerous lesions(Kappavalues were 0 .546 ,0 .628 and 0 .349 respectively) .The receiver operating characteristic curve analysis results showed that the area under the curve of p16 ,Ki-67 and CK7 combined detection was higher than that of single detection of these 3 indexes(0 .978 vs .0 .930 ,0 .966 ,0 .837) ,the difference was statistically significant (P＜0 .05) .Conclusion The expression of p16 ,Ki-67 and CK7 has the correlation with the degree of cervical precancerous lesions .Their joint detection is helpful to improve the diagnostic efficiency of cervical precancerous lesions and could be used in the assisted diagnosis of cervical lesion and prediction of high risk progress cervical precancerous lesion .