Study on Bushen Jianpi Recipe in treating bone marrow hematopoietic suppression mouse model caused by cyclophosphamide / 国际检验医学杂志

ABSTRACT

Objective To verify the effects of Bushen Jianpi Recipe(BSJP) on bone marrow hematopoietic suppression mouse model caused by cyclophosphamide .Methods The experimental C57BL/6 mice were divided into the normal control group ,bone marrow hematopoietic suppression group and BSJP treatment group ,and the bone marrow hematopoietic suppression group and BSJP treatment group were peritoneally injected with cyclophosphamide for 3 d .The BSJP group began to be given with BSJP by gavage from 4 d ,and the other two groups received water as gavage control .After 14 d treatment ,peripheral venous blood cells count ,bone marrow hematopoiesis stem cells (HSCs) count and bone marrow mononuclear cell proliferation ability were detected by using the blood cells analyzer and flow cytometry .Results Compared with the control group ,the white blood cells(WBCs) count , HSCs number and bone marrow mononuclear cell proliferation ability in the hematopoietic suppression group were decreased significantly(P＜0 .01);the platelet count ,HSCs count and bone marrow mononuclear cells proliferation ability after BSJP treatment in the BSJP treatment group were significantly higher than those in the hematopoietic suppression group (P＜0 .01) .Conclusion BSJP can alleviate the side effect of cyclophosphamide chemotherapy and increase the anti-tumor effect of chemotherapeutic drugs .