Expression Levels of Serum LCN1 and LCN2 in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease and Its Clinical Significance / 现代检验医学杂志

ABSTRACT

Objective To investigate the expression level of serum LCN1 and LCN2 in patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)and its clinical significance.Methods From May 2015 to April 2017 in Shanghai Yangpu Hospital Affiliated to Tongji University 93 cases with chronic obstructive pulmonary disease were collect-ed as the research object,the acute exacerbation of chronic obstructive pulmonary disease(COPD)in 53 patients,was as the exacerbation group,and chronic obstructive pulmonary disease(COPD)in 40 patients,was as stable group.Another 40 cases were selected to hospital for physical examination,volunteers were set as normal control group.Venous blood was collected, patients sera by ELISA kit for detection of LCN1,LCN2 and high sensitive C reactive protein(hs-CRP),correlation analysis between LCN1,LCN2 and clinical parameters of patients respectively.Results The exacerbation group,stable group and normal control group with age,gender,BMI had no significant difference(F/χ2=1.599~2.028,all P>0.05).In the exac-erbation group,the expression of hs-CRP,neutrophil percentage,and white blood cell count were significantly increased,but FEV1/FVC,FEV1% pred and PaO2were significantly decreased(F=64.942~481.808,all P<0.05).The serum levels of LCN1 and LCN2 in the exacerbation group were significantly higher than those in the stable group(t=19.088,18.904,all P<0.05).The expression of LCN1,LCN2 in serum of COPD patients with age,sex,BMI had no significant correlation(r=0.010~0.161,all P>0.05),it showed a significant positive correlation with hs-CRP,neutrophil percentage,white blood cell count(r=0.418~0.712,all P<0.05),and there was a negative correlation with FEV1/FVC,FEV1% pred and PaO2(r=-0.482~-0.782,P<0.05).Multiple linear regression analysis showed that the percentage of hs-CRP,the percentage of neutrophils,WBC count,and FEV1/FVC,FEV1% pred were independent factors of LCN1,LCN2(OR=0.329~4.035,P<0.05).Conclusion Both LCN1 and LCN2 are highly expressed in serum of patients with COPD,and the expression of LCN1 and LCN2 was closely related to the acute attack of COPD.It would be speculated that LCN1 and LCN2 may be po-tential molecular marker for the diagnosis of acute exacerbation of COPD.