Study on the Purification Technology of Total Saponins from Asparagus cochinchinensis with Macroreticu-lar Resin / 中国药房
China Pharmacy
;
(12): 4868-4871, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-663587
ABSTRACT
OBJECTIVE:
To optimize the purification technology of total saponins from Asparagus cochinchinensis with macro-reticular resin.METHODS:
Using content of total saponins from A. cochinchinensis as index,single factor test was used to investi-gate the macroreticular resin model,sampling adsorption time,mass concentration of the column,adsorption capacity,volume frac-tion and the amount of elution solvent,elution rate,and optimize the purification technology. And verification test was conducted.RESULTS:
HPD-300 macroreticular resin showed strong absorption and desorption property. The optimal purification technology was that sampling adsorption time was 60 min,mass concentration of sample liquid was 0.1 g/mL,adsorption capacity was 120 mL(15 BV),it was eluded with 60% ethanol solution with 3 BV and elution rate was 4 BV/h. In the verification test,the average desorption rate of total saponins was 68.30%(RSD=0.95%,n=3).CONCLUSIONS:
Optimized purification technology is sta-ble,feasible,and can easily separate and purify the total saponins from A. cochinchinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Pharmacy
Year:
2017
Type:
Article
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