PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone / 法医学杂志
Journal of Forensic Medicine
;
(6): 514-515,521, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-663673
ABSTRACT
Objective To establish a convenient and rapid method for extracting DNA from bone.Methods Fifteen long bone samples were washed and sterilized.The skeletal fragments were obtained by electric drill,and lysed by PrepFiler Express BTATM lysis buffer.DNA was then manually extracted by silicon microbeads for further analysis.Results STR genotyping was successfully obtained in 14 out of the 15 samples,and the detection rate was 93.33%.Conclusion The method for DNA extraction from bone established in present study is convenient,quick,effective,and with a strong applicability,which is worth spreading and applying.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2017
Type:
Article
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