Expression of miR-143 in patients with PTC and its influence on immune function / 中国免疫学杂志

ABSTRACT

Objective:To investigate the relationship between the expression level of miR 143 and prognosis/cellular biological characteristics in patients with papillary thyroid carcinoma (PTC),and explore the potential mechanisms.Methods:30 PTC patients were admitted and enrolled into the trial from 2013 April to 2016 December.The qPCR was performed to detect the expression of miR143 in cancer tissues and adjacent tissues from 30 PTC patients.The effect of miR143 on overall survival rate and metastasis-free survival rate was assessed with specific analysis .CON and pGenesil1-miR143 vector were transfected into PTC cell line IHH-448h after qPCR.CKK8 and Annexin V/PI flow cytometry were performed to detect cell proliferation ,and apoptosis,respectively.Cell migration was asseessed by transwell experiment .Levels of miR143,IL-10,IFN-γin serum were assessed and calculated with the pearson coeffi-cient.PTC patients were assigned into miR 143 high and low expression group according to miR 143 expression , and IL-10 and IFN-γwere measured with routine protocol.Results:The relative expression of miR143 in paracancerous tissues and tumor tissues was (1.0± 0.15) vs (0.12±0.06).miR143 was significantly associated with the survival rate and metastasis rate of patients (P<0.001).miR143 expression levels in IHH-4 pGenesil1-miR143 and control group were ( 8.63 ±0.71 ) vs ( 1.0 ±0.06 ) , and the proliferative index in pGenesil1-miR143 group was significantly lower than CON group (P<0.01).Percentage of Annexin and V+positive cells in pGenesil1-miR143 and CON group was (40±7.2) vs (2±0.38),and the ratio of the number of migrating cells was (40±7.2) vs (2±0.38) (P<0.001).Analysis showed that r2 were -0.4 and 0.62 for IL-10 and IFN-γ,respectively(P<0.001).In miR143 low group and high ex-pression group,expression levels of IFN-γwas (8±0.23) vs (11.2±0.12),while IL-10 was (12±3.1) vs (8.43±0.44) (P<0.05). Conclusion:miR143 is low expressed in PTC patients and associated with prognosis and survival .Highly expressed miR143 inhibited the proliferation of IHH-4 cell lines,increased apoptosis and decreased migration ,also the expression of miR143 is related to the immune function of the patients .