Effects of miR-125b on proliferation and invasion of hepatocarcinoma cells by ART4 protein / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of miR-125 b on ART4 protein and its effect on proliferation and invasion of hep-atocellular carcinoma cells .Methods:The expression of miR-125 b in hepatocellular carcinoma and hepatocellular cells was detected by qPCR.The expression of miR-125b and ART4 was detected by qPCR after overexpression of miR-125b.The expression of miR-125b and ART4 was detected by double luciferase assay .The effect of miR-125 b on the proliferation of hepatoma cells was detected by MTT assay.The effect of miR-125b on the invasion of hepatoma cells was detected by Transwell invasion assay .MTT assay was used to detect the effect of miR-125 b on the proliferation of hepatoma cells after overexpression of ART 4.Transwell invasion assay was used to detect the effect of miR-125 b on the invasion of hepatoma cells after overexpression of ART 4.Results: The expression of miR-125 b in hepatoma cells was low,and overexpression of miR-125b could inhibit the expression of ART4 protein.Overexpression of miR-125b could inhibit the proliferation and invasion of hepatoma cells .Overexpression of ART4 could reverse the proliferation and invasion of hepatoma cells by miR-125b.Conclusion:Expression of miR-125b in hepatocellular carcinoma was down-regulated.Meanwhile,miR-125 b can regulate the expression of ART 4 and affect the proliferation and invasion of hepatoma cells .