Effects of oat β-glucan on the expression of small intestine injury-related genes in septic rats / 中国比较医学杂志

ABSTRACT

Objective To explore the effect of oatβ-glucan on gene expression in small intestine injury in sepsis. Methods SD rats were randomly divided into normal control group ( NC group) , experimental control group ( TC group) and oat β-glucan group ( Oglu group) . TC group and Oglu group were used to establish sepsis model with CLP method ,and oglu group with different doses of oatβ-glucan. After 12 h the small intestine tissue were collected. Western blot was used to detect gene expression in the small intestine tissue injury. Results 1 ) Oat β-glucan reduced sepsis-induced small intestine injury,and reduced the TNF-α, IL-1βand IL-6 levels in small intestine of septic rats. (2) The expression of small intestinal injury-related proteins such as Bax, caspase-3, Toll-like, apoptotic genes FAS and FASL and NF-κB were significantly increased,and Bcl-2 expression was down-regulated in the TC group. (3) The expressions of Bax, caspase-3, Toll-like, apoptotic genes FAS and FASL and NF-κB in oat β-glucan-treated rats were lower than that in the septic rats, while Bcl-2 expression was higher than the TC group. Conclusions Oat β-glucan regulates the expression of small intestinal injury genes in septic rats and protects the small intestinal epithelium against sepsis-induced injury.