Detection of Atropine Sulfate by Surface Enhanced Raman Spectroscopy / 中国药师
China Pharmacist
;
(12): 2277-2281, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-664078
ABSTRACT
Objective:
To establish a surface enhanced Raman spectroscopy ( SERS) technique for the determination of atropine sulfate.Methods:
The Raman peaks of atropine sulfate molecules were classified by theoretical calculations and experimental tests, and the 1002 cm-1 Raman peaks were selected as the characteristic peaks for the quantitative analysis.Results:
The detection limit of atropine sulfate in aqueous solution was below 0. 5 μg·ml-1 . The relationship between the intensity of characteristic peaks at 1002 cm-1 and the concentration of aqueous solution was linear within the range of 1-8 μg ml-1 with the linear correlation coefficient r of 0. 9839. The recovery rates of 2, 5 and 7μg ml-1 were measured, which were 97. 1%-109. 8%. The average recovery was 103. 3%, and the RSD was 4. 5% (n=9). At the same time, the stability of the method among the batches was tested, and the relative standard deviation was 5. 7 %. In addition, the atractylodes rhizome samples containing atropine sulfate were detected,and the characteristic peaks still could be detected at 1002 cm-1 .Conclusion:
The method is rapid, accurate and nondestructive with easy operation, which can be used for the detection of atropine sulfate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Pharmacist
Year:
2017
Type:
Article
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