Development of Neutralization Assay using Murine Leukemia Virus (MuLV) Pseudotyped with Japanese encephalitis Virus (JEV) env Gene
Journal of Bacteriology and Virology
;
: 23-30, 2007.
Article
in Korean
| WPRIM
| ID: wpr-66408
ABSTRACT
The envelope (E) glycoprotein of JEV is the major antigen to elicit neutralizing antibody (NAb) against JEV infection. In order to develop a rapid and safe neutralization assay system for evaluation of the JEV vaccine strains, we constructed JEV-pseudotyped viruses with JEV env genes (Nakayama-NIH, Beijing-1). The titers of JEV-pseudotyped viruses with NK and BJ strains were 4.0x10(4) IFU/ml and 1.3x10(5) IFU/ml in Vero cell cultures, respectively. We have analyzed the neutralization activity of immunized mouse sera with JEV-NK and JEV-BJ pseudotyped viruses. The neutralizing antibody titers of NK and BJ (50% reduction of virus) were about 110,000 at each immunized sera. Compared with conventional plaque reduction neutralization test (PRNT), the method using JEV-pseudotyped virus has desirable advantages such as more rapid, easier, and non-biohazardous. This neutralization assay system might be useful to evaluate NAb activity against JEV vaccine strains or vaccine candidates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vero Cells
/
Neutralization Tests
/
Glycoproteins
/
Genes, env
/
Encephalitis, Japanese
/
Leukemia Virus, Murine
/
Asian People
/
Encephalitis Virus, Japanese
/
Antibodies, Neutralizing
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2007
Type:
Article
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