Rapidly-identification of anti-inflammation candidates targeting Nuclear Receptor Retinoid X receptor-α from Epimedium brevicornum Maxim / 国际检验医学杂志
International Journal of Laboratory Medicine
;
(12): 3245-3248, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-664080
ABSTRACT
Objective To establish a method that can accurately and efficiently screen potential pharmaceutical agents targeting Nuclear Receptor Retinoid X receptor-α (RXRα) from Traditional Chinese Medicine .Methods Based on the principle of Receptor-Ligand Recognition ,the fusion protein of His-RXRαLBD and the active fractions were mixed to incubate for capturing and identif-ying potential ligand of RXRα,and then the identified ligand was evaluated for RXRα-dependent anti-inflammation effect .Results Baohuoside Ⅰ from Epimedium brevicornum Maxim .was identified as a RXRα binder ,and could induce RXRα-dependent anti-in-flammation effect .Conclusion The method was an efficient strategy to accurately identify active compounds from natural products , to expedite the discovery of natural RXRαregulator ,and also to share the thought with other receptors in identifying ligand .
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
International Journal of Laboratory Medicine
Year:
2017
Type:
Article
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