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Simultaneous Determination of Three Components in Xiaoji Huachong Powder by UPLC / 中国药师
China Pharmacist ; (12): 2258-2260, 2017.
Article in Chinese | WPRIM | ID: wpr-664089
ABSTRACT

Objective:

To establish a method for simultaneously determining 3 components ( hesperidin, magnolol and honokiol) in Xiaoji Huachong powder by UPLC.

Methods:

The experiments were performed on a Thermo Accucore C18 column ( 150 mm × 4. 6 mm, 2. 6 μm). The mobile phase was methanol-0. 5% glacial acetic acid in water with gradient elution, the flow rate was 0. 7 ml ·min-1 , the column temperature was maintained at 30 ℃,the detection wavelength was set at 283 nm and the injection volume was 2μl.

Results:

The 3 constituents showed a good linear relationship within the range of 4. 707-28. 242 μg·ml-1(r=0. 9994) for hes-peridin, 5. 085-30. 510 μg·ml-1(r=0. 9997) for magnolol and 10. 651-63. 908 μg·ml-1(r=0. 9995) for honokiol. The average recovery was 101. 2%,104. 8% and 99. 4% with the RSDs of 1. 11%,1. 68% and 1. 49% (n=6), respectively.

Conclusion:

The experiment provides a simple and effective method that can be used to evaluate the quality of Xiaoji Huachong powder.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacist Year: 2017 Type: Article