AICAR promotes the wound healing in diabetic ulcers by improving the survival rate of MSC in biomaterials transplantation / 局解手术学杂志

ABSTRACT

Objective To investigate the mechanism of AICAR in improving the survival of mesenchymal stem cells (MSCs) in biomaterials and the therapeutic effect on diabetic ulcer.Methods Totally 40 male C57BL/6J mice were equally divided into 4 groupscontrol group,MSCs group,AICAR group and AICAR-MSCs group.The effects of AICAR on the apoptosis and migration of MSCs were observed by flow cytometry and transwell migration assay.The expression of VEGF in conditioned medium was detected.Prepared diabetic foot ulcer model and AICAR-stimulated MSCs-covered wounds.The wound neovascularization was observe by CD31 staining 2 weeks later.Results Compared with the control group and MSCs group,the results of the cell experiments showed that the AICAR-MSCs group had lower MSCs apoptosis rate,better MSCs migration ability and higher VEGF secretion.Animal experiments showed that AICAR-stimulated MSCs covered diabetic foot ulcer wounds had good angiogenesis and rapid ulcer healing processes.Conclusion AICAR promotes the survival and paracrine effect of MSCs by inhibiting the high glucose effect on MSCs apoptosis,so as to promote the angiogenesis and accelerate the healing of ulcer.