Research progress of lens culture procedure / 中华实验眼科杂志

ABSTRACT

There are various methods to study lens disorders,mainly in vivo and in vitro.In vitro,besides lens epithelial cells culture,lens tissue culture has been widely used,which helps to evaluate onset risk and explore pathogenesis in scientific research field.Especially in pharmacology,lens tissue culture has manifested its superiority as a screening test that helps to estimate both ocular toxic and protective effects of a certain drug and screen out appropriate drug prospectively and retrospectively.In addition,models constructed by tissue culture reveal pathophysiology of lens more intuitively and their culture conditions are much easier to control.However,methods for sampling,culture and identification were diverse.Therefore,this review aimed to retrospect the front research of lens tissue culture both at home and aboard in biological property,sampling,tissue culture,identification of condition and application.