Expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions / 实用医学杂志

ABSTRACT

Objective To investigate the expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions. Methods Cervical tissues were selected from normal cervical(NC),low-grade cervical intraepithelial lesion(LSIL),high-grade cervical intraepithelial lesion(HSIL)and cervical squa-mous cell carcinoma(SCC)from each thirty patients.The expression of DNMT1 and CCNA1 mRNA and protein was examined by Western Blot analysis and qRT-PCR in cervical tissues. Results The expression of DNMT1 mRNA and protein in LSIL,HSIL and SCC was higher than in NC(F=117.93,P<0.05;F=61.24,P<0.05). Expression of DNMT1 mRNA and protein was increased steadily according to severity of cervical lesions(χ2trend=26.25,P<0.05;χ2trend=26.60,P<0.05).The expression of CCNA1 mRNA and protein in HSIL and SCC was lower than that in NC and LSIL(F = 77.04,P < 0.05;F = 57.15,P < 0.05). Expression of CCNA1 mRNA and protein was decreased steadily according to severity of cervical lesions(χ2trend=64.19,P<0.05;χ2trend=60.24,P<0.05). There was a negative correlation between expression of DNMT1 and CCNA1 protein in LSIL,HSIL,SCC (r=-0.75,-0.56,P<0.05). Conclusion In DNMT1 mRNA there is high expression of protein and in CCNA1 mRNA there is low expression of protein,but both may be related to the occurrence and development of cervical cancer.