Effect and mechanism study of PSRC1 overexpression on foam cell formation of RAW264.7 / 实用医学杂志

ABSTRACT

Objective To investigate the effect and potential mechanism of PSRC1 overexpression on foam cell formation of oxidized low-density lipoprotein(ox-LDL)-induced RAW264.7. Method After 48-h treat-ment of 100 μg/ml ox-LDL,changes of the accumulation of cholesterol esters in two groups was detected by oil red O staining.The protein expression of SR-AⅠand LDLR was detected by Western blot assay.Besides,ELISA was used to detect levels of IL-6 and TNF-α.Result The accumulation of cholesterol esters was lower in Ad-PSRC1 group than that in Ad-GFP group(P<0.05). The protein expression of SR-AⅠand LDLR was decreased signifi-cantly(P<0.05),and levels of the secreted IL-6 and TNF-α were also significantly decreased(P<0.05).Con-clusion Our data indicates that PSRC1 overexpression suppresses the formation of foam cells through improving lipid metabolism and down-regulating inflammatory cytokine IL-6 and TNF-α in macrophages.