Expression and clinical significance of MEG3 and GAS5 in the colorectal cancer / 中国现代普通外科进展

ABSTRACT

Objective:To detect the expression of long non-coding RNA (Long non-coding RNA,IncRNA) maternally expressed gene 3 (maternally expressed gene 3,MEG3),specific growth inhibitor 5 (Growth arrest-specific 5,GAS5) and to analyze the correlation between GAS5 and cyclin P21,E2F1,so as to investigate the mechanism of GAS5.Methods:From December 2014 to December 2015,63 consecutive Patients with colorectal cancer admitted to Qingdao Municipal Hospital for surgical treatment were included into our study.We collect colorectal cancer tissues and paired normal tissues.We tested the relative expression of MEG3,GAS5 genes by real-time quantitative PCR (RT-PCR),and Western blot was used to detect the expression of P21 and E2F1 in colorectal cancer and to evaluate their correlations with GAS5.Results:The expression of MEG3 in cancer (7.76 ± 1.38) was lower than that in normal tissues(9.52 ± 1.31)P＜0.052.The expression of GAS5 was decreased in cancer tissues (3.98 ± 1.15) relative to the normal (6.21 ± 1.33)P＜0.05.3.It showed that there were positive relationships between P21 and GAS5(r=0.410,P=0.001),and there were negative relationships between E2F1 and GAS5(r=-0.366,P=0.003).Conclusions:MEG3 and GAS5 were decreased in colorectal cancer,suggesting that they play inhibiting effects on the cancer.P21 and E2F1 are important target spots that GAS5 give play to the role of tumor suppressor.