Relationship between abnormal glycosylation of serum IgA1 and oxidative stress in patients with primary IgA nephropathy / 中国医师进修杂志

ABSTRACT

Objective To study the relationship between abnormal glycosylation of serum IgA1 and oxidative stress in patients with primary IgA nephropathy (IgAN).Methods Fifty-two primary IgAN patients diagnosed by renal biopsy from January 2012 to December 2013 were selected.In the patients,Lee gradeⅠ-Ⅲand estimated glomerular filtration rate(eGFR)≥60 ml/min 41 cases(IgAN-A group),Lee grade≥Ⅲand eGFR<60 ml/min 11 cases(IgAN-B group).Sixty-nine health examination volunteers were selected as control group.The serum galactose-deficient IgA1(Gd-IgA1)was detected by enzyme-linked immunosorbent assay for vicia lectin, the advanced oxidation protein products (AOPPs) was detected by liquid chromatography.Results The urinary protein,urea nitrogen,creatinine and uric acid in IgAN-B group were significantly higher than those in IgAN-A group(3 613 ± 2 247)mg/d vs.(1 457 ± 1 342)mg/d,(8.30 ± 2.92)mmol/L vs.(5.46 ± 1.55)mmol/L,(155.09 ± 57.97)μmol/L vs.(77.50 ± 22.55)μmol/L and(466.73 ± 120.97)μmol/L vs.(361.70 ± 94.40)μmol/L,the albumin and eGFR were significantly lower than those in IgAN-A group(36.45 ± 4.71)g/L vs.(39.37 ± 3.97)g/L and (50.71 ± 26.50) ml/min vs.(94.67 ± 23.48) ml/min, there were statistical differences (P<0.05).There were no statistical differences in IgG, IgA, IgM, C3and C4between IgAN-A group and IgAN-B group(P>0.05).The serum Gd-IgA1 and AOPPs in IgAN group were significantly higher than those in control group0.44 ± 0.17 vs.0.15 ± 0.06 and(120.3 ± 48.5)μmol/L vs.(45.7 ± 19.2)μmol/L,there were statistical differences (P<0.05).The serum Gd- IgA1 and AOPPs in IgAN- B group were significantly higher than those in IgAN-A group0.59 ± 0.23 vs.0.35 ± 0.12 and(159.4 ± 50.2)μmol/L vs.(90.9 ± 41.7) μmol/L, there were statistical differences (P<0.05).There was a positive correlation between Gd-IgA1 and AOPPs in IgAN group (r = 0.603, P<0.05).Conclusions Gd-IgA1 may up-regulate oxidative stress.The serum levels of Gd-IgA1 and AOPPs are related to histopathological change of IgAN, which suggests that Gd-IgA1 combined with AOPPs may become a new biomarker for prognosis of IgAN.