Simultaneous determination of five constituents in Maxing Kanggan Granules by HPLC / 中成药

ABSTRACT

AIM To establish an HPLC method for the simultaneous content determination of five constituents in Maxing Kanggan Granules (Isatidis Radix,Houttuyniae Herba,Scutellariae Radix,etc.).METHODS The analysis of 70％ ethanol extract of this drug was performed on a 30 ℃ thermostatic Wondasil C18 column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 245,327,280 and 250 nm.RESULTS (R,S)-goitrin,chlorogenic acid,baicalin,baicalein and glycyrrhizic acid showed good linear relationships within the ranges of 0.24-6.05 μg/mL (r =0.999 9),0.70-17.40 μg/mL (r =0.999 9),28.59-714.70 μg/mL (r =0.999 6),1.67-41.65 μg/mL (r =0.999 5) and 2.59-64.80 μg/mL (r =0.999 7),whose average recoveries were 99.53％,99.41％,98.66％,98.31％ and 99.25％ with the RSDs of 1.02％,1.05％,1.26％,0.85％ and 1.43％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Maxing Kanggan Granules.